Fig. 2.

The kinetics of OCT2-mediated transport of MPP (A), metformin (B), TEA (C), cimetidine (D), NBD-MTMA (E), and ASP (F). Rates of uptake presented here were corrected for the first-order component of transport (see eq. 1 and Supplemental Fig. 1). Each data point represents the mean ± S.E. rate of mediated (saturable) transport based on 30-second (MPP, metformin, TEA, and cimetidine) or 2-minute (NBD-MTMA and ASP) net accumulation in five to eight replicate wells with 8–12 different substrate concentrations, determined in three to eight individual experiments. Lines and kinetic parameters were derived from fitting the Michaelis-Menten equation to these data.