Table 3.
Hazard Ratios for the Association of Metabolite Risk Score With All-Cause Mortality in the 30% Test Sets in the Alpha-Tocopherol, Beta-Carotene Cancer Prevention Study, Finland, 1985–2013
| Quartile of Metabolite Risk Scorea | Event | Participant | Person-years | HRb | 95% CI |
|---|---|---|---|---|---|
| 1 | 27 | 44 | 690.4 | 1.00 | Referent |
| 2 | 25 | 41 | 570.7 | 1.30 | 0.71, 2.38 |
| 3 | 36 | 45 | 474.7 | 1.94 | 1.08, 3.48 |
| 4 | 37 | 43 | 400.6 | 1.73 | 0.91, 3.30 |
| P value for trend | 0.05 | ||||
| Metabolite risk score as continuous variable (per SD) | 1.38 | 1.08, 1.75 |
Abbreviations: CI, confidence intervals; FDR, false discovery rate; HDL, high-density lipoprotein; HR, hazard ratios; SD, standard-deviation.
a The risk score was generated by summing the top 12 metabolites (FDR of ≤0.1) identified in the test set, which were weighed by their regression coefficients in the training set. The risk score is calculated as 0.073 × (log-N-acetylvaline) + 0.085 × (log-C-glycosyltryptophan) + 0.07 × (log-dimethylglycine) + 0.084 × (log-N-acetylputrescine) + 0.077 × (log-erythronate) + 0.07 × (log-ADSGEGDFXAEGGGVR) + 0.071 × (log-mannose) + 0.082 × (log-taurochenodeoxycholate) + 0.065 × (log-taurocholate) + 0.095 × (log-5,6-dihydrothymine) + 0.078 × (log-N-acetylmethionine) + 0.085 × (log-acisoga).
b Hazard ratio for all-cause mortality generated from Cox proportional hazards regression, and adjusted for age at randomization, body mass index, number of cigarettes per day, history of diabetes, serum cholesterol, HDL, history of hypertension (elevated blood pressure), serum creatinine, and metabolomic sets.