Table 2.
Relative P450 Protein Levels in Hepatic Microsomes Prepared From Dams Exposed to PCB 95 or PCB 136 and Their P21 Pups (Relative OD/mg Microsomal Protein)a,b
| Group | Cyp1a2 | Cyp2b |
Cyp3a |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cyp2b10 |
Cyp2b9 |
Cyp3a11 |
Cyp3a41 |
Cyp3a13/25 |
||||||||
| Upper band | Lower band | Upper band | Middle band | Lower band | ||||||||
| PUPS | ||||||||||||
| Female | Male | Female | Male | Female | Male | Female | Male | Female | Male | Female | Male | |
| Vehicle (n♀ = 4, n♂ = 4) | 76 ± 13 | 63 ± 10 | 5.7 ± 1.3# | 3.1 ± 1.1 | 11 ± 2# | 7 ± 2 | 12 ± 3 | 11 ± 2 | ND | ND | 3.2 ± 0.8 | 2.9 ± 0.6 |
| PCB 95 (n♀ = 5, n♂ = 5) | 75 ± 25 | 82 ± 21 | 9.4 ± 3.2* | 7.1 ± 2.1* | 13 ± 5 | 10 ± 3 | 11 ± 4 | 12 ± 5 | ND | ND | 3.5 ± 1.4 | 3.6 ± 1.7 |
| PCB 136 (n♀ = 6, n♂ = 6) | 84 ± 13 | 93 ± 7*$ | 8.9 ± 2.0*$ | 6.6 ± 2.4*$ | 17 ± 3*# | 12 ± 3 | 15 ± 3$ | 14 ± 2$ | ND | ND | 3.4 ± 1.2 | 2.9 ± 0.6 |
| DAMS | ||||||||||||
| Vehicle (n = 5) | 69 ± 15 | 23 ± 5 | 17 ± 3 | 6.7 ± 0.1 | 16 ± 1 | 6.6 ± 1.1 | ||||||
| PCB 95 (n = 5) | 62 ± 3 | 21 ± 2 | 14 ± 1 | 5.5 ± 0.9* | 14 ± 2 | 5.4 ± 0.9 | ||||||
| PCB 136 (n = 6) | 64 ± 17 | 18 ± 3* | 14 ± 3 | 3.6 ± 1.1** | 13 ± 1** | 4.6 ± 0.9* | ||||||
a
Relative OD is defined as the optical density (ie, contour quantity) of the P450 immunoreactive band/optical density of the internal standard band. Representative immunoblots are shown in Supplementary Figs. 7-9.
b
One female and one male pup from each litter were analyzed. Values are presented as mean ± SD. ND, not detected.
Significantly different from the corresponding pups or dams from the control group, *P < .05; **P < .01.,
Significantly different from their respective dams, $P < .05.
Significantly different from the corresponding male pups in the same exposure group, #P < .05.