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. 2017 Apr 14;158(1):101–115. doi: 10.1093/toxsci/kfx071

Table 3.

Changes in Expression of Neural Plasticity and Thyroid Hormone-Responsive Genes in the Cerebellum, Cortex, and Hippocampus From P21 Pups of Dams Exposed to PCB 95 or PCB 136, Relative to Age- and Sex-Matched Vehicle Control Animals

Comparison arc
mbp
nrgn
ppp1r9b
Relative expression 95% CI Relative expression 95% CI Relative expression 95% CI Relative expression 95% CI
PCB 95 Cb Female 1.4 0.59–7.5 0.60a ↓ (P = .003) 0.15–1.0 3.4 0.65–158 1.2 0.19–4.4
Male 0.67 0.37–1.2 0.61 0.31–1.1 0.78 0.16–4.9 1.1 0.59–4.0
Ctx Female 2.0 0.11–17 0.42 0.07–1.8 1.1 0.01–19 0.99 0.08–6.7
Male 2.0 0.51–17 0.58 0.17–1.6 1.3 0.35–34 1.3 0.23–13
Hippo Female 0.62 0.24–1.5 0.82 0.26–2.1 0.70a ↓ (P = .006) 0.53–0.94 0.70 0.12–3.5
Male 1.3 0.53–2.8 1.1 0.32–2.7 0.79a ↓ (P = .006) 0.60–1.0 1.9 0.35–7.4
PCB 136 Cb Female 0.56a ↓ (P = .03) 0.29–1.5 0.57a ↓ (P = .02) 0.33–1.2 2.1a ↑ (P = .02) 0.93–5.9 1.3 0.27–4.2
Male 0.69 0.36–1.5 0.67a ↓ (P = .05) 0.37–1.4 0.39 0.06–2.8 1.3 0.55–5.1
Ctx Female 2.9a ↑ (P = .04) 0.59–21 0.7 0.33–1.6 2.7 0.61–20 3.8a ↑ (P = .006) 0.98–12
Male 1.2 0.15–13 1.3 0.49–2.8 1.0 0.21–28 2.0a ↑ (P = .04) 0.82–12
Hippo Female 0.83 0.35–2.1 1.0 0.49–2.0 0.80a ↓ (P = .02) 0.60–1.1 1.1 0.52–3.1
Male 1.2 0.43–2.6 1.6 0.61–3.6 0.85 0.62–1.2 2.6a ↑ (P = .05) 0.43–9.3
a

Indicates statistically significant change (↑: upregulation; ↓: downregulation) of gene expression (P < .05). The relative expression (shown in bold) is calculated as described before in Stamou et al. (2014, 2015), followed by the P-value in parentheses. Gene transcript levels were normalized against the geometric mean of 3 reference genes (pgk1, ppia, and hgprt). Relative expression, P-values and 95% CIs were calculated using the REST2009 software (Qiagen), which incorporates Ct and efficiency values determined by qPCR analysis. Arc, activity-regulated cytoskeleton-associated protein; Cb, cerebellum; Ctx, cortex; Hippo, hippocampus; hprt, hypoxanthine-guanine phosphoribosyl transferase; mbp, myelin basic protein; nrgn, RC3/neurogranin; pgk1, phosphoglycerate kinase 1; ppia, peptidylprolyl isomerase A; ppp1r9b, spinophilin.