Table 3.
Comparison |
arc |
mbp |
nrgn |
ppp1r9b |
||||||
---|---|---|---|---|---|---|---|---|---|---|
Relative expression | 95% CI | Relative expression | 95% CI | Relative expression | 95% CI | Relative expression | 95% CI | |||
PCB 95 | Cb | Female | 1.4 | 0.59–7.5 | 0.60a ↓ (P = .003) | 0.15–1.0 | 3.4 | 0.65–158 | 1.2 | 0.19–4.4 |
Male | 0.67 | 0.37–1.2 | 0.61 | 0.31–1.1 | 0.78 | 0.16–4.9 | 1.1 | 0.59–4.0 | ||
Ctx | Female | 2.0 | 0.11–17 | 0.42 | 0.07–1.8 | 1.1 | 0.01–19 | 0.99 | 0.08–6.7 | |
Male | 2.0 | 0.51–17 | 0.58 | 0.17–1.6 | 1.3 | 0.35–34 | 1.3 | 0.23–13 | ||
Hippo | Female | 0.62 | 0.24–1.5 | 0.82 | 0.26–2.1 | 0.70a ↓ (P = .006) | 0.53–0.94 | 0.70 | 0.12–3.5 | |
Male | 1.3 | 0.53–2.8 | 1.1 | 0.32–2.7 | 0.79a ↓ (P = .006) | 0.60–1.0 | 1.9 | 0.35–7.4 | ||
PCB 136 | Cb | Female | 0.56a ↓ (P = .03) | 0.29–1.5 | 0.57a ↓ (P = .02) | 0.33–1.2 | 2.1a ↑ (P = .02) | 0.93–5.9 | 1.3 | 0.27–4.2 |
Male | 0.69 | 0.36–1.5 | 0.67a ↓ (P = .05) | 0.37–1.4 | 0.39 | 0.06–2.8 | 1.3 | 0.55–5.1 | ||
Ctx | Female | 2.9a ↑ (P = .04) | 0.59–21 | 0.7 | 0.33–1.6 | 2.7 | 0.61–20 | 3.8a ↑ (P = .006) | 0.98–12 | |
Male | 1.2 | 0.15–13 | 1.3 | 0.49–2.8 | 1.0 | 0.21–28 | 2.0a ↑ (P = .04) | 0.82–12 | ||
Hippo | Female | 0.83 | 0.35–2.1 | 1.0 | 0.49–2.0 | 0.80a ↓ (P = .02) | 0.60–1.1 | 1.1 | 0.52–3.1 | |
Male | 1.2 | 0.43–2.6 | 1.6 | 0.61–3.6 | 0.85 | 0.62–1.2 | 2.6a ↑ (P = .05) | 0.43–9.3 |
Indicates statistically significant change (↑: upregulation; ↓: downregulation) of gene expression (P < .05). The relative expression (shown in bold) is calculated as described before in Stamou et al. (2014, 2015), followed by the P-value in parentheses. Gene transcript levels were normalized against the geometric mean of 3 reference genes (pgk1, ppia, and hgprt). Relative expression, P-values and 95% CIs were calculated using the REST2009 software (Qiagen), which incorporates Ct and efficiency values determined by qPCR analysis. Arc, activity-regulated cytoskeleton-associated protein; Cb, cerebellum; Ctx, cortex; Hippo, hippocampus; hprt, hypoxanthine-guanine phosphoribosyl transferase; mbp, myelin basic protein; nrgn, RC3/neurogranin; pgk1, phosphoglycerate kinase 1; ppia, peptidylprolyl isomerase A; ppp1r9b, spinophilin.