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. 2018 Apr 19;1(2):e201800059. doi: 10.26508/lsa.201800059

Figure 3. The β-tubulin C-terminal tail modulates microtubule assembly in an isotype-dependent manner.

Figure 3.

(A) Representative images of EB3-transfected gene-edited cells (i). The image processing involved in measuring the assembly parameters: image appearance following the processing of raw images (ii), identification of microtubule plus ends by the particle tracking analysis (iii), and microtubule assembly events colored according to assembly rate (iv). Scale bar 5 μm. (B) Microtubule assembly parameters in gene-edited NCI-H460 cells expressing the full-length βIII-tubulin protein (ZB3), truncated βIII-tubulin protein (ZB3Δ), and βIII-tubulin body with βI-tubulin tail (ZB3/CB1) as measured by EB3-mCherry motion and particle tracking. The microtubule assembly rate (i), microtubule growth length (ii), microtubule growth duration (iii), and the number of microtubule growth events (iv) were calculated as the average value per cell and are presented as the per-cell mean ± SEM of at least 50 cells in each of three independent experiments for each tubulin modification. *P < 0.05, ***P < 0.001, and ****P < 0.0001 relative to cells expressing the full-length protein (ZB3).