Figure S1. Modification of the β-tubulin C-terminal tail does not affect the tubulin isotype composition.

(A) Expression of the modified β-tubulin proteins in gene-edited cells expressing the full-length βIII-tubulin protein (ZB3), truncated βIII-tubulin protein (ZB3Δ), and βIII-tubulin body with βI-tubulin tail (ZB3/CB1) compared with the parental cell line using antibodies against GFP (upper blot) and S55 of βIII-tubulin (middle blot) with GAPDH as a loading control. Representative Western blot of two independent experiments. (B) Quantitation of the Western blot in (A) for two independent experiments. Graph gives the mean ± SEM. (C) Western blot of the β-tubulin isotype expression in gene-edited NCI-H460 cells. The protein expression of the βI-, βII-, βIII-, and βIV-tubulin isotypes together with the expression of the modified tubulin proteins (GFP) and total tubulin levels for each clone are shown. The GFP, βIII-tubulin and GAPDH Western blots are replicated in Fig 1B.