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. 2018 Apr 19;1(2):e201800059. doi: 10.26508/lsa.201800059

Figure S4. The β-tubulin C-terminal tail affects microtubule assembly measured by spatiotemporal image correlation spectroscopy analysis.

Figure S4.

(A) Schematic outline of spatiotemporal image correlation analysis showing time-lapse images are acquired of cells transiently transfected with EB3 (i); time-lapse images are subdivided into overlapping voxels (ii); the autocorrelation function of GFP, autocorrelation function of EB3, or the cross-correlation function of EB3 with GFP is calculated and the velocities extracted per voxel (iii); and voxels are shifted in space and time to produce a vector map indicating directional GFP and EB3 movement (iv). (B) Measures of microtubule motion by spatiotemporal image correlation spectroscopy. (i) Speed of directional microtubule motion as measured by the autocorrelation function for tubulin-GFP time-lapse images of gene-edited cells expressing modified βIII-tubulin proteins. (ii) Speed of microtubule assembly as measured by the autocorrelation function for EB3-mCherry time-lapse images of gene-edited cells expressing modified βIII-tubulin proteins. (iii) Number of microtubule growth events as measured by the autocorrelation function for EB3-mCherry time-lapse images of gene-edited cells expressing modified βIII-tubulin proteins. Graphs give the median, box gives the 25th to the 75th percentile, and whiskers give the minimum and maximum values of at least 15 cells from 2 independent experiments. **P < 0.05 and **P < 0.01 relative to cells expressing the full-length βIII-tubulin protein.