Fig 4. Reproducibility of CLMCLA and FLAPF measurements in blood samples.
(a) Time courses of CLMCLA and FLAPF signals from blood samples in four independent experiments. CFL-P2200 was used for the measurements. The light colors indicate that the order of the measurement was later. The left vertical axis shows CLMCLA intensity; the right vertical axis, FLAPF intensity. PMA was added at 150 second (dotted line). The baseline heights of FLAPF signals were adjusted for making the four independent experiments start at the same level. (b) Calculated signal areas measured in (a). Increases in both CLMCLA and FLAPF were determined by calculating AUCs. Relative errors of the four measurements were 7.06% for CLMCLA and 7.94% for FLAPF.