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. 2018 Aug 1;13(8):e0201024. doi: 10.1371/journal.pone.0201024

Fig 7. Expression analysis of JcARF genes in leaves in response to IAA, carried out by qRT-PCR.

Fig 7

The fourth leaves of 25-day-old seedlings grown at 25°C under 16/8 h (light/dark) conditions in a growth chamber were incubated for 0 h, 1 h, 6 h or 12 h in either MS nutrient solution (control) or MS nutrient solution containing 15 μM indole-3-acetic acid (IAA). The JcActin gene was used as an internal control. The experiment included three biological replicates, each with two technical replicates. Values represent means of n = 6 ± SD (Duncan test: **, P < 0.01; *, P < 0.05).