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. 2018 Aug 1;13(8):e0201609. doi: 10.1371/journal.pone.0201609

Fig 4. Phenotypic characterization of dSETD3 KO flies.

Fig 4

(A) Wing size of adult female control (n = 7) and dSETD3 KO (n = 8) flies. Error bars: standard deviation (SD). (B) Weight of control and dSETD3 KO flies (n = 4 x 8 flies for each condition). Error bars: standard deviation (SD). (C) Pupation timing of control and dSETD3 KO animals at 25°C (n = 8 x 30 flies for each condition). Error bars: standard deviation (SD). (D) Fat storage measured by TAG abundance in control and dSETD3 KO flies, normalized to total body weight (n = 4 x 8 flies for each condition). Error bars: standard deviation (SD). (E) Glycogen storage measured in control and dSETD3 KO flies, normalized to total body weight (n = 4 x 8 flies for each condition). Error bars: standard deviation (SD); * t test < 0.05. (F-F’) Full starvation of control and dSETD3 KO animals on PBS/agarose (0.7%) for male (F) or female (F’) adult flies (n = 3 x 20 flies for each condition). Error bars: standard deviation (SD). (G) Fertility of control and dSETD3 KO mated females over the course of three days (n = 4 x 8–10 flies). Error bars: standard deviation (SD); * t test < 0.05. (H) dSETD3 KO flies do not have impaired motility, assayed using a climbing assay with control and dSETD3 KO adult females. Flies were put into plastic tubes, tapped down, and observed climbing towards a light source at the top of the tube. The time was measured that was required for 50% of the flies in one tube to pass a set threshold (biological quadruplicates, each measured twice). Error bars: standard deviation (SD).