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. 2018 Aug 1;4(8):eaat5107. doi: 10.1126/sciadv.aat5107

Fig. 3. Controllable in vitro expression of diverse fluorescent proteins.

Fig. 3

FD-CF reactions were rehydrated with 25, 10, 5, 2.5, or 0 ng of template DNA encoding mCherry, mRFP1, dTomato, mOrange, or YPet and run for 20 hours at 30°C. (A) Results from experiments run by graduate students (experts), high school students, or middle and high school teachers are shown. In all cases, we observed a concomitant decrease in protein synthesis as the amount of DNA template was decreased. Values represent averages, and error bars represent average errors of n ≥ 2 biological replicates. (B) The variation in protein expression was marked enough to be observed qualitatively with the naked eye under both white light and blue light. Images are representative examples of experiments prepared by high school students.