Figure 1. Graphical representation of the experimental procedures that were performed. A highly sensitive proteomic technology of liquid chromatography coupled to an Orbitrap mass analyzer (LC-MS/MS) was applied in the secretome analysis of cell lines. Furthermore, a systematic bioinformatics analysis of the differentially expressed proteins, identified in the proteomics analysis, revealed significant pathways involved in cervical cancer pathogenesis, such as the inhibition of matrix metalloproteases. Finally, the multiple putative targets identified, were further validated by independent methods such as zymography, western blot analysis and the targeted proteomic method of multiple reaction monitoring (MRM).