Figure 6. Western blot analysis of the secretome for SOD2. A. A specific band of 22 kDa was detected. B. Graphical representation of western blot analysis for each cell line (mean±SD; *p<0.05, Student’s t-test). C. LC/MS-MS results confirmed by western blot analysis. The results of LC/MS-MS are presented by fold change compared to HCK1T (mean±SD; *p<0.05, Mann Whitney) as well as the western blot analysis data (mean±SD; *p<0.05, Student’s t-test). Representative images of two biological replicates are shown for each cell line. Equal loading of the secretome was confirmed by Coomassie Colloidal Blue-stained gels, or by Ponceau S- stained membranes.