Figure 1. Schematic illustration of the Autoantibody Profiling System workflow for screening of plasma. A: Plasma from nine donors diagnosed with breast cancer was used as the test group. Three samples were pooled to obtain three test samples (T1, T2, and T3). B: Plasma from nine donors with no history of breast cancer was used as the reference (R) group. Three samples were pooled to obtain three reference samples (R1, R2, and R3). Only the screening of sample T2 and R2 are illustrated. A total 50 μl of each pooled sample was used for the assay. A total of six mini-APS plates were used for the study. All incubations and washes were performed at ambient temperature and the assay lasted for about 4 h.