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. Author manuscript; available in PMC: 2019 Jul 5.
Published in final edited form as: Vaccine. 2018 Jun 7;36(29):4346–4353. doi: 10.1016/j.vaccine.2018.05.092

Fig. 1.

Fig. 1

Preparation and characterization of recombinant baculovirus (rBV) for expression of multi-clade H5N1 VLPs. (a) Location and order of Bgag, N1 and H5 genes in the rBV. Polyhedrin promoters are schematically indicated with arrows. Clades of H5 genes are also shown. (b) Detection of H5 in the VLPs prepared by using passages 1-5 of rBV. Passages of rBV used for expression of VLPs are indicated by numbers 1 to 5. VLPs produced by rBV were harvested from Sf9 growth medium and probed by western blot (left panel) and HA assay with 0.5% turkey RBCs (right panel). M, protein molecular weight marker SeeBlue Plus 2 (Thermo). Western blot was done using H5-specific mouse IgG2a monoclonal antibody (H5N1) IT-003-005M6. HA titers prepared using 2-fold dilutions of VLPs are also indicated.