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. Author manuscript; available in PMC: 2019 Jun 15.

Published in final edited form as: J Immunol. 2018 May 11;200(12):3942–3949. doi: 10.4049/jimmunol.1800259

(A) Expression pattern of AIRE, MHC-2, Tg, and TSHR in parental (mixed) GD-OF and pure CD34⁺ OF and CD34⁻ OF subsets. Parental GD-OF strains were subjected to sham cytometric sorting or sorted into CD34⁺ OF and CD34⁻ OF subsets and cultured for 3 d. (B) Conditioned media were generated by incubating GD-OF , CD34⁺ OF , and CD34⁻ OF in DMEM for 48 h. These media were then used to cover fibrocytes for 3 d. Monolayers were harvested, RNA extracted, reverse-transcribed and subjected to real-time PCR. Data are expressed as the mean ± SD of 3 independent replicates. Values were normalized to their respective GAPDH levels and are expressed as mean ± SD of triplicate determinations. *** p<0.001; ** p<0.01. Experiments were performed three times.

Inline graphic — (A) Expression pattern of AIRE, MHC-2, Tg, and TSHR in parental (mixed) GD-OF and pure CD34⁺ OF and CD34⁻ OF subsets. Parental GD-OF strains were subjected to sham cytometric sorting or sorted into CD34⁺ OF and CD34⁻ OF subsets and cultured for 3 d. (B) Conditioned media were generated by incubating GD-OF , CD34⁺ OF , and CD34⁻ OF in DMEM for 48 h. These media were then used to cover fibrocytes for 3 d. Monolayers were harvested, RNA extracted, reverse-transcribed and subjected to real-time PCR. Data are expressed as the mean ± SD of 3 independent replicates. Values were normalized to their respective GAPDH levels and are expressed as mean ± SD of triplicate determinations. *** p<0.001; ** p<0.01. Experiments were performed three times.