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. Author manuscript; available in PMC: 2019 Jun 15.
Published in final edited form as: J Immunol. 2018 May 11;200(12):3942–3949. doi: 10.4049/jimmunol.1800259

Figure 5.

Figure 5

rhSlit2 attenuates expression of AIRE, MHC-2, Tg and TSHR in fibrocytes. (A) Cultured PBMCs were treated with rhSlit2 (50ng/mL) for 7–9 d, fibrocyte monolayers were harvested, stained with the antibodies indicated and subjected to flow cytometric analysis. (B) Fibrocytes were untreated or treated with rhSlit2 and then some were treated with bTSH for 6 h, monolayers harvested, RNA was extracted, reverse-transcribed, and subjected to real-time PCR for the targets indicated. Values were normalized to their respective GAPDH levels and β-actin levels and are expressed as the mean ± SD of triplicate determinations. ** p<0.01. Experiments were performed three times.