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. Author manuscript; available in PMC: 2019 Sep 28.
Published in final edited form as: Cancer Lett. 2018 Jun 15;432:169–179. doi: 10.1016/j.canlet.2018.06.006

Figure 2.

Figure 2

Cell cycle was arrested at G0/G1 phase in KYSE-150 cells treated with RP4010. (A) Hoechst 33342 (blue) and PI (red) staining of KYSE-150 cells treated with vehicle (control), RP4010 (10 µM), or MK2206 for 48 hours. MK2206, an Akt inhibitor was used as positive control for cell death. (B) The populations of RP4010 treated KYSE-150 cells in G0, G1 and S phase were calculated by flow cytometry.(C) Statistical data of the percentage of G0/G1, S and G2/M phase in the KYSE-150 cells with RP4010 treatment at the concentration of 0, 0.4, 2 and 10 µM. (D) Western Blot of cell cycle related proteins, i.e. Cyclin B1, Cyclin D1 and P27 were performed in KYSE-150 cells treated with RP4010 at concentrations of 0, 0.4, 2 and 10 µM, respectively. GAPDH was used as a loading control. Data is showed as mean ± SD. **p ≤ 0.01 and *p ≤ 0.05.