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. Author manuscript; available in PMC: 2019 Sep 3.
Published in final edited form as: Biochem Biophys Res Commun. 2018 Jun 6;503(1):51–55. doi: 10.1016/j.bbrc.2018.05.170

Figure 2. AURKA is associated with elongated spermatids and spermatozoa in the seminiferous epithelia.

Figure 2

(A-H) Frozen testis sections were stained for AURKA (red in A and D) and DAPI (blue in B and E) with merged images in C and F. Images were taken with a 40X objective (A-C, G) or a 100X objective (D-F, H). In tubules containing abundant elongating spermatids, AURKA was associated with the heads of elongating spermatids during the process of spermiogenesis (arrowheads, D and F). (G-H) AURKA also localized to the flagella of sperm within the lumen of the tubule (arrowheads in H). These are the flagella of immotile sperm undergoing spermiation. Negative control incubated without primary antibody is shown in I. Scale bars indicate 10μm.