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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Am Chem Soc. 2018 Jul 24;140(30):9478–9485. doi: 10.1021/jacs.8b03572

Figure 3.

Figure 3

Selective antibody recognition of hydrazide mimics. (A) Antibody against H3-K56ac (WB: immunoblot; CB: Colloidal Blue visualization of the loading control). (B) Pan 2-hydroxyisobutyryl-Lys antibody. (C) Ubiquitin antibody. H3-unmod refers to the unmodified human H3.1 harboring C96S, G102A, and C110A mutations. Full gels of loading controls stained with Colloidal Blue corresponding to Figure 3A–C are shown in Supplementary Figure 10A–C, respectively. Pan-2-hydroxyisobutyryl-Lys antibody recognizes the modification in H3 better at Kc122 than at Kc56, and that same observation was made in each replicate.