Figure 4.

EGcpB and EcpC positively regulate pelD gene expression via different pathways. (A) The promoter activity of pelD was examined in Dickeya dadantii. (B) RNA levels of rsmB and hns were examined using quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). The mutant/wild‐type (WT) ratio of each gene was calculated as described in Experimental procedures. (C) Northern blot analysis of rsmB mRNA in D. dadantii strains. (D) mRNA levels of pelD in mutant strains relative to that in the wild‐type strain. Values are representative of three independent experiments. Three replicates were used in each experiment. Error bars indicate standard errors of the means. Asterisks indicate statistically significant differences of the means (P < 0.05 by Student's t‐test).