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. 2018 Aug 1;9:3012. doi: 10.1038/s41467-018-05429-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Generation of Plk1-inducible mice. a Schematic representation of the alleles used in this work. A cassette containing the human FLAG-Plk1 cDNA downstream of the tetO sequences is inserted in the endogenous ColA1 locus after homologous recombination in KH2 ES cells. This allele [ColA1(Plk1) or (Plk1) in short] is later combined with the Rosa26-rtTA allele expressing the tetracycline transactivator. b (+/Plk1);(+/rtTA) ES cells were treated with Dox and FLAG and Plk1 signal was detected using specific antibodies at the indicated time points. Vinculin was used as a loading control. c Immunofluorescence of (+/Plk1);(+/rtTA) ES cells treated with Dox for 12 h. FLAG (green) is concentrated at the spindle poles with some signal in the spindle microtubules and additional diffuse signal as expected for Plk1. α-tubulin is in red and DAPI in blue. Scale bar 5 μm. d Immunodetection of Flag-Plk1 in the indicated tissues from (+/+);(rtTA/rtTA); (+/Plk1)(+/rtTA) and (+/Plk1)(rtTA/rtTA) mice treated with Dox for 8 weeks. Scale bar 100 μm. e Tumor-free survival of (+/+);(+/rtTA) and (+/Plk1)(+/rtTA) mice fed with Dox since birth during 85 weeks. (+/+)(+/rtTA), 19 mice; (+/Plk1)(+/rtTA), 24 mice. p = 0.2836; Log-rank (Mantel–Cox) test. f Sections of Dox-treated (+/+);(+/rtTA) and (+/Plk1)(+/rtTA) mice after staining with hematoxylin and eosin (H&E) or immunodetection of Plk1 (right panel). Cells with abnormally large nuclei are indicated with arrows. Black scale bar: 100 μm, blue scale bar: 50 μm