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. 2018 Aug 1;8:11552. doi: 10.1038/s41598-018-29884-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SAXS- and HDX-analysis of the FliS/flagellin/FliW interaction. (a) Size-exclusion chromatograms (SEC) of flagellin/FliS (red), FliW (blue) and FliS/flagellin/FliW (green). The inset shows a Coomasse-stained SDS PAGE of the peak fractions. (b) SAXS analysis of FliW, flagellin/FliS and FliS/flagellin/FliW. The crystal structures have been fitted to the SAXS-density with the docking algorithm implemented in Chimera. (c) HDX analysis of flagellin/FliS versus flagellin/FliW. The difference in HDX-labelling is shown from blue (less exchange) to red (more exchange) in Dalton. Blue regions in the cartoon representation of flagellin/FliS exchange less in the presence of FliW (F1 and F2), whereas red regions get unprotected. (d) HDX analysis of FliW versus flagellin/FliW. The difference in HDX-labelling is shown from blue (less exchange) to red (more exchange) in Dalton. Blue regions in the cartoon representation of FliW exchange less in the presence of flagellin (W1 and W2). (e) SEC-chromatogram of a flagellin_N72/FliW complex (left) and a Coomassie-stained SDS-PAGE of the two peak fractions (right).