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. 2018 Aug 1;8:11570. doi: 10.1038/s41598-018-30047-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of CTCs and identification of potential HCC driver genes. (A) Volcano plot of differential gene expression between the 3 CTCs found in the 2 patients and the rest of blood cells. Red dots denote comparisons with an FDR < 0.05. (B) Bar size represent normalized enrichment score values and FDR (i.e., red gradient) for gene sets significantly enriched in each CTC resulting from GSEA. (C) Expression of ASGR1 on scRNAseq in non-CTC blood cells. Scaled expression depicted as a red gradient (grey denotes no expression detected for ASGR1).