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. 2018 Aug 1;9(8):825. doi: 10.1038/s41419-018-0887-0

Fig. 2. PAK2 affects proliferation of head and neck cancer cells.

Fig. 2

PAK2 expression was depleted in two types of HNC cell lines (H157 and H413), and differential proliferation status of these cell lines was analyzed. a, e Knockdown of PAK2 expression was analyzed at mRNA and protein levels (n = 3). b, f Relative cell proliferation was analyzed through MTT assay (n = 3). c, g mRNA and d, h protein expression status of cell proliferation marker Ki67 (left) was analyzed through qPCR and flow cytometer, respectively. (Right) Mean fluorescence intensity (MFI) of Alexa Fluor 488-Ki67 was measured using software FlowJo 10 (n = 3). i, k (Left) Cell-cycle stages were analyzed using flow cytometer (representative image of 3 independent experiment) (right) bar graph shows average of three independent experiments and j, l cyclin D1 (CCND1) protein expression level was analyzed through immunoblotting (n = 3). Abs absorbance. Samples in (a–c) and (e–g) were analyzed in duplicate. Error bars shows mean values ± SD. Differences were considered statistically significant with *P < 0.05, **P < 0.01 and ***P < 0.001, ns non-significant difference (P > 0.05)