Fig. 6. PAK2 depletion from head and neck cancer cells globally affects the expression of proliferation genes.

The PAK2 was depleted in H157 cells and total mRNA was isolated for global analysis, human transcriptome array (HTA) 2.0. a The mRNA expression of PAK2 was analyzed by qRT-PCR upon knockdown. b Globally differentially expressed genes were analyzed through volcano plot upon PAK2 depletion. c Heat map of top 10 up- and downregulated genes in head and neck cancer cells upon PAK2 knockdown. d The mRNA expression of differentially expressed genes, serum amyloid A1 (SAA1), family with sequence similarity 72 member D (FAM72D), baculoviral IAP repeat containing 3 (BIRC3), anillin actin binding protein (ANLN), keratin 1 (KRT1), aquaporin 3 (AQP3), TNF superfamily member 10 (TNFSF10) and matrix metallopeptidase 13 (MMP13) were verified by quantitative real-time PCR and e Gene Ontology analysis of downregulated genes upon PAK2 depletion. Error bars shows mean values ± SD. Differences were considered statistically significant with *P < 0.05, **P < 0.01 and ***P < 0.001