Fig. 1.

Deletion mapping of D. melanogaster DmYP1 and P2C-mediated delivery of EGFP. a Schematic showing the construct pAc5-STABLE1-neo backbone modified to express and secrete EGFP fused to DmYP1 or any of its derivatives (P2, P3–P4, P5, or P6) under the Actin5C promoter in S2 cells. Fragments P2, P3, and P4 are about 120 amino acids of DmYP1 each. Portion P5 contains portions P2 and P3. Portion P6 contains P3 and P4. b EGFP fusion proteins containing DmYP1 or each fragment (P2–P6) as ligand were injected into the hemolymph of vitellogenic A. gambiae females. Ovaries were dissected 16 h after injections. Fragments containing the N-terminal region of DmYP1 showed the highest transduction of EGFP into the ovaries. c Deletion analysis of the P2 region. EGFP was detected at the highest intensity when fused to the P2C portion of DmYP1. Scale bar is 100 µm