Fig. 2.

P2C-mediated protein translocation in several mosquito species. a P2C-EGFP, expressed in E. coli using the plasmid pET28a (See details in Supplementary Fig. 3), was injected into the hemolymph of several species of vitellogenic mosquitoes. Negative control injections were performed with unmodified EGFP. Scale bars: A. stephensi—50 µm; A. freeborni—500 µm; C. tarsalis, A. albopictus, A. aegypti—100 µm. b Merged images of fluorescence detected in immunofluorescence assays on ovaries of A. aegypti females injected intrathoracically with different P2C-Cas9 proteins. P2C-Cas9 and P2C-EGFP-Cas9 showed distinctive granules (white arrows) containing Cas9-; these granules are co-localized with EGFP in P2C-EGFP-Cas9. From left to right: Cas9 (No ligand), P2C-Cas9, P2C-EGFP-Cas9. Blue = DAPI, Red = Cas9 antibody, Green = EGFP, Yellow = overlap in red and green signal. Scale bars = 50 µm