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. 2018 Aug 1;8:11527. doi: 10.1038/s41598-018-29874-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bovine primary miR-21 exists as two transcript variants and contains a conserved putative upstream promoter region. (A) Predicted pri-miR-21 transcript variants based on RACE-PCR derived sequences. (B) DNA gel electrophoresis of RT-PCR amplified pri-miR-21 transcript variants in bovine tissues and cells. A very faint band representing pri-miR-21 transcript variant 1 in testis is detectable. NTC; no template control. (C) Bos taurus pri-miR-21 variant 1 plus 1 kb upstream region; alignment to Mus musculus Ch11 and Homo sapiens Ch17. Major ticks on x-axis = 1000 nucleotides, minor ticks = 500 nucleotides. Blue = coding exon, salmon = intronic region, yellow = untranslated region, red = intergenic region. Height of conservation plot at each position represents the number of nucleotides conserved out of a 100 nucleotide window centered at that position. Horizontal pink bars denote evolutionarily conserved regions (ECR) to the bovine reference genome. ECR defined as a minimum 70% identity over 100 nucleotide regions. Vertical hatched line denotes pri-miR-21 transcription start site.