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. 2018 Aug 1;8:11527. doi: 10.1038/s41598-018-29874-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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STAT3 signaling modulates the expression of pri-miR-21 in bovine cumulus cells and STAT3 inhibition disrupts cumulus expansion in vitro. (A) Representative Western blot of STAT3 phosphorylation (Y705) and inhibition by Stattic in cumulus cells from 0–8 hr IVM. Shown with total STAT3. Beta-actin is used as a loading control. Cropped images of a single blot are shown. Full-length blots are presented in Supplemental Fig. 2. Three independent experiments were performed with similar results. (B) qRT-PCR of pri-miR-21 at 0 and 7 hr IVM in cumulus cells in control media, DMSO vehicle (VEH) and 10 μM Stattic (STT). Gene expression is shown relative to the 0 hour time point. Error bars represent SEM. N = 3 biological replicates. Different letters indicate statistically significant differences between groups. (C) Light micrographs of immature (0 hr) and mature (24 hr) COCs matured in DMSO vehicle (VEH) or 10 μM Stattic (STT). Images obtained with EVOS FL Cell Imaging System using 4x objective.