Table 2.
Specific primer sequences used for RACE PCR, cloning and site directed mutagenesis.
| Primer name | Primer sequence (5′-3′) |
|---|---|
| RACE | |
| pri-miR-21 5′RACE Gene Specific Primer pri-miR-21 3′RACE Gene Specific Primer |
GCTGCTGGATTCGTTTGGCGT TCATGGCAACAGCAGTCGATGG |
| pri-miR-21 Cloning | |
| pri-miR-21 Cloning Forward pri-miR-21 Cloning Reverse |
TGAGCTCGCCTCACTCTGAGAACT AATGCGGGTGAAGGTGATGACAGAC |
| miPPR-21 Cloning | |
| miPPR-21 KpnI Forward miPPR-21 BglII Reverse |
GATCGGTACCTGCGTGTTTTGAA GATCAGATCTGAATGACTTCTGAGAAG |
| miPPR-21 STAT3 Site Directed Mutagenesis | |
| miPPR-21-41GΔC-40AΔG Forward miPPR-21-41GΔC-40AΔG Reverse |
GTTCAAACCAGTCCTTACAGCGACTGGTGGTGATAAATGTGA TCACATTTATCACCACCAGTCGCTGTAAGGACTGGTTTGAAC |
| miPPR-21-9AΔG-8AΔC Forward miPPR-21-9AΔG-8AΔC Reverse |
GTGATAAATGTGAGACTTCTCAGCGGTCATTCAGATCTGCGATCTAAG CTTAGATCGCAGATCTGAATGACCGCTGAGAAGTCTCACATTTATCAC |