Table 1.
Reverse genetics virus | HACS motif a | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
HA1 | HA2 | ||||||||||||
P12 | P11 | P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 | P1’ | |
rgHPAIV | N | S | P | Q | R | E | R | R | R | K | K | R | G |
rgLPAIV | N | S | P | Q | R | E | — | — | — | — | T | R | G |
Mutant 5 | N | S | P | Q | R | E | — | — | — | — | K | R | G |
Mutant 6 | N | S | P | Q | R | — | — | — | — | K | T | R | G |
Mutant 7 | N | S | P | Q | — | — | — | — | R | K | K | R | G |
Mutant 4 | N | S | P | Q | R | E | — | — | R | K | K | R | G |
Mutant 8 | N | S | P | Q | — | — | — | R | R | K | K | R | G |
Mutant 9 | N | S | P | Q | R | E | — | R | R | K | K | R | G |
Mutant 2 | N | S | P | Q | R | E | R | R | R | K | T | R | G |
Mutant 1 | N | S | P | Q | R | E | R | R | Q | K | K | R | G |
Mutant 3 | N | V | P | Q | R | E | R | R | R | K | K | R | G |
1Dash indicates deletion, underlined indicates mutation. Cleavage occurs C-terminally at the scissile bond between P1 (HA1) and P1’ (HA2). The HACS motif is flanked N-terminally by PQ, and C-terminally by G.