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. 2018 Aug 1;8:11547. doi: 10.1038/s41598-018-30058-9

Figure 2.

Figure 2

N. brasiliensis L3 antigen decreases HPV negative and positive cervical cancer cell migration. (A) Scratch motility assay showing Ca Ski cells exposed to 0.1 µg, 1 µg or 10 µg N. brasiliensis L3 antigen and imaged at 4 and 8 hours post-wound formation and the total area migrated calculated relative to wound area at time 0 hours. (B) Scratch motility assay showing C33-A cells exposed to 0.1 µg, 1 µg and 10 µg N. brasiliensis L3 antigen imaged at 4 and 8 hours post-wound formation. (C) Transwell migration of C33-A cells determined at 24 hours following exposure to 10 µg N. brasiliensis L3 antigen or an untreated control. (D) Western blot analysis of N-cadherin, vimentin and p38 (loading control) was performed 12 or 24 hours following exposure of C33-A cells to 10 μg or 50 µg N. brasiliensis L3 antigen or an untreated control and (E). Densitometry readings were obtained using ImageJ in order to determine the changes in protein expression of N-cadherin and vimentin normalised to p38 and expressed as a fold-change relative to control wells. The figures shown are the result of (A–C). Three independent experiments, (E). Two independent experiments, or (D). Representative of two independent experiments. The data was analysed using GraphPad Prism 6.01 and a parametric unpaired t-test (A–C) or two-way ANOVA (D) was performed where ns = not significant, *p < 0.05 **p < 0.01 and ***p < 0.001. Error bars represent Standard Error of the Mean.