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. 2018 Aug 1;8:11536. doi: 10.1038/s41598-018-29813-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Signal-to-noise for candidate food dyes in Con-Ex. Data are the summed absorbance values of INT + ExVial. Dye (simple filled bars): absorbance values from flies fed media containing 1% of the indicated dyes (signal). No Dye (hatched bars): absorbance values from flies fed media without dye (noise). Control r[A] female flies (20/vial) consumed media and excreted waste products in vials for 24 h. Numbers in brackets indicate signal-to-noise ratios. Signal (Dye) was greater than noise (No dye) in experiments with (panel A) Blue 1, xylene cyanol (XC) and Red 40, and (panel B) Blue 2, Red 4 and Yellow 5 (*individual two-tailed t tests, p = 0.007 to <0.0001, n = 5–6).