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. 2018 Aug 1;8:11536. doi: 10.1038/s41598-018-29813-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Composition of food media influences Con-Ex. Control r[A] females consumed the indicated media labeled with 1% Blue 1 and excreted waste for 24 h. ExVial + INT was greater in flies that consumed 0.25X medium than in flies fed 1.0 × 2Y10S3C medium (panel A, *two-tailed t test, p < 0.0001, n = 16), in flies fed medium containing 2% yeast (2Y10S3C) compared to 30% yeast (30Y10S3C) (panel B, *two-tailed t test, p < 0.0001, n = 8) and in flies fed medium containing 10% sucrose (2Y10S3C) compared to 30% sucrose (2Y30S3C) (panel C, *two-tailed t test, p = 0.0003, n = 8). (D) ExVial + INT was indistinguishable in flies fed medium containing 3% cornmeal (2Y10S3C) and 10% cornmeal (2Y10S10C) (two-tailed t test, p = 0.2532, n = 8).