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. 2018 Aug 1;8:11536. doi: 10.1038/s41598-018-29813-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CAFE consumption of Blue 1 equates with excretion of Blue 1. (A) In control r[A] females, the volume of liquid medium consumed from capillary feeders (CAFE) and excreted (ExVial + ExPlug) was significantly increased by starvation, but the two measures were statistically indistinguishable (two-way ANOVA; starvation, p < 0.0001; measure, p = 0.5302, interaction, p = 0.3537; n = 18–24 per group). (B) Additional analysis of the data in panel A. The volume of liquid medium consumed from capillary feeders (CAFE) correlated with ExVial, ExPlug and ExVial + ExPlug (Pearson correlations: R² = 0.6573, R² = 0.8165, R² = 0.9539, p < 0.0001 for all, n = 42). Lines are best-fit linear regressions.