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. 2018 Aug 1;8:11577. doi: 10.1038/s41598-018-29966-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microscopic findings. Some of the most relevant microscopic findings revealed by scansion electron microscopy (SEM), histopathology, and immunohistochemistry (IHC) examinations: (A) evidences of post-mortem bacteria proliferation and (B) post-mortem degeneration of Organo del Corti outer hear cells (OHC, white arrows) observed at SEM. (C) occasional fat emboli within pulmonary capillary vessels of SW3 revealed by OsO4 post-fixation technique, PAS 20×. (D) Positive immunostaining of axons using anti-caspases-3 antibody, suggesting ongoing apoptotic changes in in SW2’s brain; 40×. (E) IHC on muscular tissues revealed a multifocal cytoplasmic immunostaining within fibers by using anti-fibrinogen antibody suggesting an ongoing damage (40×); (F) rabdomyolisis was further confirm by IHC on kidneys using anti-myoglobin antibody: the picture shows myoglobin granules in the apical side of tubular epithelial cells (40×); (G) positive IHC staining of circulating monocytes in the spleen of SW3 by using anti-CDV antibody (40×); (H) this image shows the same results of the previous image in dendritic cells of SW3’s spleen (arrows), 40×.