Figure 5.

Enzyme linked immunosorbent assay (ELISA) of iron uptake proteins or OprH. (A–D) FpvA, FoxA, HasR, or mixture of iron uptake proteins were used to coat plates. The ELISAs were conducted using sera from mice immunized with each of the iron uptake proteins or the combination of these proteins with curdlan or curdlan alone. (E) Sera from mice immunized with the refolded OprH or DHPC alone with curdlan were tested for antigen-specific IgG levels by ELISA with plates coated with OprH. (F) ELISA of the whole bacterial cells. The cells of PA14 or the ΔoprH mutant were coated on an ELISA plate. Sera from mice immunized with refolded OprH or DHPC alone with curdlan were tested for the levels IgG that can bind to the cells by ELISA. Each point is the average of duplicates using pooled sera from five to seven mice, and error bars are SDs.