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. 2018 Jul 26;9:1728. doi: 10.3389/fimmu.2018.01728

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Copyright © 2018 Neidert, von Ehr, Zöller and Spittau.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of Olfml3 as a transforming growth factor β1 (TGFβ1)-regulated gene. (A) Scheme illustrating the process of mRNA isolation from adult microglia of Cx3cr1^CreERT2:Tgfbr2^fl/fl transgenic mutant mice. (B) Expression of Olfml3 in adult wild-type (+/+TAM) and Tgfbr2 mutant microglia (Cre/+TAM). Immunohistochemistry to monitor expression of Olfml3 in cortical microglia of wild-type (+/+TAM) (C) and Tgfbr2-deficient (Cre/+TAM) mice (D). Scale bars indicate 10 µm. (E) Scheme summarizing TAM-induced recombination and isolation of postnatal microglia in vitro isolated from P0/P1 mice. (F) Significantly decreased expression of Olfml3 in Tgfbr2-deficient (Cre/+TAM) postnatal microglia recombined in vitro. Data are presented as mean ± SEM from at least three independent experiments (B,F). P-value derived from one-way ANOVA is **P < 0.01.