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. 2018 Jul 26;9:1683. doi: 10.3389/fimmu.2018.01683

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Copyright © 2018 Wang, Hu, Wangkahart, Liu, Wang, Zahran, Maisey, Liu, Xu, Imarai and Secombes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rainbow trout IL-2 isoforms promote proliferation in peripheral blood leukocytes (PBL). Freshly prepared PBL from four fish were incubated with IL-2A and IL-2B, or with medium alone as control in triplicate for 3 days. BrdU was added 20 h before incorporation of BrdU was detected by ELISA. The data are presented as the average (+SEM) stimulation index, calculated as the OD450 of IL-2-treated cells divided by that of untreated samples. Different letters over bars indicate significant differences (p ≤ 0.05, paired samples T-test).