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. 2018 Jul 26;9:1683. doi: 10.3389/fimmu.2018.01683

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Copyright © 2018 Wang, Hu, Wangkahart, Liu, Wang, Zahran, Maisey, Liu, Xu, Imarai and Secombes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree analysis of salmonid IL-2, IL-2 and IL-2L from other fish species, and selected mammalian IL-2 molecules with the closely related γC cytokines IL-15 and IL-21. The phylogenetic tree was constructed using amino acid (aa) multiple alignments and the neighbor-joining method within the MEGA7.0 program (36). The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (10,000 replicates) is shown next to the branches. The evolutionary distances were computed using the JTT matrix-based method with all ambiguous positions removed for each sequence pair. The aa sequences and accession numbers are detailed in Supplementary Material: protein sequence.