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. 2018 Jul 26;9:1683. doi: 10.3389/fimmu.2018.01683

Figure 8.

Figure 8

Modulation of T helper 1 pathway gene expression by IL-2 isoforms in peripheral blood leukocytes (PBL). Freshly prepared PBL from four fish were incubated with 200 ng/ml IL-2A, IL-2B, or both (IL-2A + IL-2B), or with medium alone as control for 4, 8, 24, and 48 h. The expression of IFNγ1 (A), IFNγ2 (B), CXCL11L1 (C), TNFα1 (D), TNFα2 (E), IL12P35A1 (F), IL12P40B2 (G) and IL12P40C (H) was quantified as in Figure 6. The data are presented as mean (+SEM) arbitrary units where one unit equals the average expression level in the control samples at 4 h. Significant results of a paired samples T-test between the stimulated samples and time-matched controls are shown above the bars as *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001. Different letters over the control bars indicate significant differences over time in the unstimulated cells (p ≤ 0.05). The expression levels in samples treated with IL-2B or IL-2A + IL-2B show no differences but are higher than IL-2A-treated samples for IFNγ1 at 8, 24, and 48 h, IFNγ2 at 8 and 48 h, and CXCL11L1 at 24 h (p ≤ 0.05).