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. 2018 Jul 5;8(8):1188–1201. doi: 10.1002/2211-5463.12482

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© 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Impaired proliferation of eIF3e ^+/− cells. (A) Microscopy images of MEFs established from eIF3e ^+/+ (+/+), eIF3e ^+/− (+/−) mice embryos. (B) Western blotting of eIF3e and GAPDH as a loading control (left) and (C) qRT‐PCR showing the eIF3e mRNA expression normalized by 18S rRNA gene (right) in the eIF3e ^+/+ and eIF3e ^+/− MEFs. Columns represent mean values with SD from two independent experiments. (D) Cell numbers and viability of eIF3e ^+/+ and eIF3e ^+/− MEFs from three clones each. Cell numbers are counted at 1, 3, and 5 days after starting to culture cells at 5 × 10⁴. Viabilities (%) calculated by trypan blue exclusion test are shown at 1, 3, and 5 days. Columns represent mean values with SD from three independent experiments. The asterisk indicates P < 0.05 when compared to eIF3e ^+/+.