Figure 2.

Assessing the effects of nicotine on neuronal excitability in the MHbV and IP in WT and α5^KO mice. A, Schematic of anterior recording site for IP neurons. B, Example traces of IPR neuron responses to nicotine in WT and α5^KO mice. C, Average IPR neuronal firing frequency across baseline, nicotine (1 μm, 1 min), and washout recording. D, Peak nicotine-elicited firing frequency is significantly larger for WT than α5^KO IPR neurons (Mann–Whitney U = 46.0, p = 0.02). E, Schematic illustrating recording sites for MHbV neurons. F, Example traces of MHbV neuron responses to nicotine at resting membrane potential in WT and α5^KO mice. G, Average MHbV neuronal firing frequency across baseline, nicotine (1 μm, 1 min) and washout recording. H, Peak nicotine-elicited firing frequency does not differ between WT and α5^KO mice in MHbV neurons at resting membrane potential (Mann–Whitney U = 49.0, p = 0.2). I, Example traces of MHbV neuron responses to nicotine in WT and α5^KO mice recorded from −70 mV. J, Average MHbV neuronal firing frequency across baseline, nicotine (1 μm, 1 min) and washout recording from −70 mV. K, Peak MHbV neuronal firing frequency does not differ between WT and α5^KO mice recorded from −70 mV (Mann–Whitney U = 58.0, p = 0.3). See Figure 2-1 (in table form). *p < 0.05.