FIGURE 5.

Confocal analysis of MCF-7 (A) and MCF-7TG (G) cells mock-treated and treated with bozepinib (B,C,H,I), FC-26c (D,J), FC-29b (E,K), and FC-30b2 (F,L). Cells were stained with annexin V-FITC and PI as described in the section “Materials and Methods.” Early apoptosis (stained positive for annexin V-FITC and negative for PI), late apoptosis or cell death (stained positive for both annexin V-FITC and PI) and necrosis (stained positive for PI) can be observed; viable cells are not visible. In MCF-7 treated cells we observe cells characteristic of late (A,B,D) and early apoptosis (C,E,F). MCF-7TG cells treated with different drugs showed some early and late apoptotic cells (G,H,J–L) but much more necrotic cells (H,I,K,L).