Figure 1.

miRNA Expression Pattern in Different Human MB and Normal Cerebellar Tissues

(A) Heatmap analysis of miRNAs in normal and MB tissues. Unsupervised hierarchical clustering analysis was performed for significantly (>2-fold change) expressed miRNAs. miRNAs are indicated in columns, and tumor samples are indicated in rows. N1, N2, N3, N4, and N5 are the normal cerebellar tissue samples; and T1, T2, T3, T4, and T5 are the MB samples. A green-to-red color scale shows an increase in normalized miRNA expression on a log scale. (B) Scatterplot of miRNAs significantly upregulated (y axis) and downregulated (x axis) in MB tissues compared to normal cerebellar tissues. The scatterplot graph was generated by averaging log₂(2^{−ΔΔ Ct}) values from real-time PCR analysis. A fold change of two or more was considered significant. Each circle corresponds to one miRNA.