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Sequential ND-FISH analysis of the metaphase chromosomes of wheat Chinese Spring. (A) Using probes Oligo-B (red) and Oligo-D (green) to analyze root tip metaphase chromosomes of wheat Chinese Spring. (B) Using probes Oligo-pTa535-1 (red) and Oligo-pSc119.2-1 (green) to analyze the same cell in (A) after rising the slide. Chromosomes were counterstained with DAPI (blue). Scale bar: 10 μm.
