Table 1.
Signal patterns of oligo probes developed in this study.
| Probe | Nucleotide sequences | Signal location on chromosome | Probe | Nucleotide sequences | Signal location on chromosome |
|---|---|---|---|---|---|
| Oligo-1AS | 5′AAAAACGCATGTCTTTAGCATTCAAAA AATGAAAAACGGTTTTTCTTGTTAAACAA3′ |
Subtelomeric region of 1AS | Oligo-7DL | 5′CATCATTTAGGTTCTTATCGACACCG AGGCACCCTAAAAGCCTAAGCTTT3′ |
Telomeric region of 7DL |
| Oligo-1AL | 5′TTCATTTTTACTCAACTCAAACTAT GGCACATACCAGGTACATCCAAA3′ |
Telomeric region of 1AL | Oligo-119 | 5′ATTTTGAGCTAGCTAAGCATATTAAGTCAT TTTGGAGCAAAAAAATGCTAAGTATAGGT3 |
Intercalary regions of 3BS, 6BL, and 7BS; pericentromeric region of 4BL |
| Oligo-4AL | 5′TAACTACATGCATGAATATCCTATCAC GCCATGAAGCTAGCTAACCAACTAACC3′ |
Intercalary region of 4AL | Oligo-18 | 5′GTAGCAGTAGCAGTAGTA3′ | Pericentromeric regions of 3BL and 7BL; centromeric region of 2B; intercalary regions of 1AL, 7AL, and 7BL |
| Oligo-5AS | 5′AACTTTTTTCAAATTCGATG3′ | Subtelomeric region of 5AS | Oligo-44 | 5′TAGCTCTACAAGCTAGTTCAAA TAATTTTACACTAGAGTTGAAC3′ |
Intercalary regions of 3AL, 5AL, and 5BL; telomeric region of 7AS; subtelomeric region of 7AL; pericentromeric region of 5DS |
| Oligo-5AL | 5′ATCTATCTATAGTGATACTCCTTTGTTT GTACTAGTAATTTTGTTTGTACCCTTTC3′ |
Telomeric region of 5AL | Oligo-42 | 5′CTCGCTCGCCCAGCTGCTGCT ACTCCGGCTCTCGCTCGATCG3′ |
Pericentromeric regions of 1AS, 6BL, and 7BL; centromeric region of 2B; intercalary regions of 3BS, 6BS, and 7BL; centromeric and pericentromeric regions of 5B |
| Oligo-7AL | 5′CCTTCTCCATCATTGGTGCCATGACG GAGATGTTCTTATCAATGTAAGAGACC3′ |
Subtelomeric region of 7AL | Oligo-45 | 5′CGGCCGCTCCGCGCGTCGCCATC GGTTGGTCACCTCATCACCACT3′ |
Pericentromeric regions of 3AS, 6AS, 4DS, 5DS, and 7DS; telomeric region of 5AL |
| Oligo-2BS | 5′GCGTGGCCAGCCACGGCGGAACCAAC CCAATCTCGCAAAAGCGACCCCATCAC3′ |
Telomeric region of 2BS | Oligo-428 | 5′AAATGAACTGGCGAGCGGAATCGTTTGGA ACACAAAGAACCATGAAACGTGACTCTCTC3′ |
Pericentromeric regions of 2AS and 5DS; intercalary regions of 1DS, 1DL, 2DL, 4DL, and 6DS; subtelomeric regions of 2DS and 5DL |
| Oligo-3BL | 5′GCCTGAAAATTACAACTTTTCGACACCGC CACGTGTAAGCCCGACCCAAACACAACCC3′ |
Telomeric region of 3BL | Oligo-440.1 | 5′CGAGAGGATCGCCGAGGGGTCGG GAGGTTGCCTAGTGTCAAAGGATTCAA3′ |
Subtelomeric regions of 2AS, 7AS, and 7AL |
| Oligo-4BS | 5′GGCGGTGGGAGTGTGTTTGTCGCGCCG CAACGCCTTCTCTGTTCCTCTACGCAC3′ |
Subtelomeric region of 4BS | Oligo-440.2 | 5′CGACCCCTCGGCGATCCTCGACCCCTCGA ACCCTCGACGACCCTGGAACCCTCGACCCC3′ |
Intercalary regions of 3BS and 5BS; pericentromeric region of 5BL |
| Oligo-5BS | 5′GTAGCAAGCATAGATGCTCTTCCACACGGT TTCTCTCAAGAACCGACCAATGCAGAAAC3′ |
Subtelomeric region of 5BS | Oligo-37 | 5′CTATATGATGAATTGCTA TGAGCTGCTCCTGCTGCTG3′ |
Pericentromeric region of 1AL; pericentromeric and intercalary regions of 7BL |
| Oligo-2DL | 5′AAGAGAATTGCTCTGTTAA TACGAATCAATGCAACCG3′ |
Intercalary region of 2DL | Oligo-60 | 5′CCTCTCCCTCTTCTCCATTGCAAAACC ACCCCCTCTCCTCTCCATCATCTCCATT3′ |
Intercalary regions of 3BS and 6BS; pericentromeric regions of 5BS and 5BL |
| Oligo-3DS | 5′CCTCGCGGGGGCGCGTCAGCGCACCCGCT GGGTGTAGCCCCCGAGATTCGGGCCGACTG3′ |
Subtelomeric region of 3DS | Oligo-107 | 5′ATTTCAGTACTGAAATACAACACCAC GATGACCCGACTAGTTCTATATCACTAA3′ |
Telomeric regions of 3DS, 4DS, and 4DL; intercalary region of 4AL |
| Oligo-3D | 5′GGCGGTTGACACCATGGCGTGGAGAT GGAGATCACCACGAGGACAACCATG3′ |
Subtelomeric region of 3DS and pericentromeric region of 3DL | Oligo-B | 5′GGTTCAGGAATAGCCT CAGGAATTGGCTCAATT3′ |
Mainly on the whole B-genome chromosomes |
| Oligo-6DS.1 | 5′TTTTAGCTCCCATTTAACACACC AAACTTTGACTTCCTTTTTCTTTT3′ |
Telomeric region of 6DS | Oligo-D | 5′TACGGGTGCCAAACGAGTGTCTGAA AGACTCCTCGAGAGGAAAATGCGAA3′ |
Mainly on the whole D-genome chromosomes |
| Oligo-6DS.2 | 5′GAAGGGCCCCACATGTCATACTCTCACAG GGGACCATGCCCCACCTGTGTCATCCCC3′ |
Pericentromeric region of 6DS |