Table 3.
Repeatability assessment using the high-throughput test.
| CV of replicates (%) | R2 of duplicates (%) | |||||
|---|---|---|---|---|---|---|
| Glycan | 2-AB | ProA | RF-MS | 2-AB | ProA | RF-MS |
| FA1 | 15.0 | 5.2 | 9.0 | 94.9 | 99.4 | 98.6 |
| A2 | 13.5 | 2.9 | 2.8 | 98.8 | 99.4 | 98.8 |
| FA2 | 1.9 | 1.6 | 2.7 | 99.5 | 99.2 | 97.0 |
| FA2B + A2[6]G1 | 4.4 | 2.0 | 2.2 | 99.1 | 98.3 | 98.6 |
| A2[3]G1 | 11.4 | 4.6 | 1.8 | 99.0 | 95.2 | 98.8 |
| FA2[6]G1 | 1.6 | 0.7 | 2.4 | 95.6 | 98.3 | 94.1 |
| FA2[3]G1 | 2.0 | 0.8 | 2.2 | 99.2 | 98.6 | 96.4 |
| FA2[6]BG1 | 1.5 | 1.4 | 1.7 | 99.5 | 99.2 | 99.4 |
| FA2[3]BG1 | 6.0 | 8.7 | 1.7 | 95.8 | 52.1 | 87.3 |
| A2G2 | 9.9 | 13.2 | 1.9 | 99.6 | 88.7 | 98.7 |
| FA2G2 + A2BG2 | 1.3 | 1.5 | 1.9 | 98.7 | 98.8 | 99.1 |
| FA2BG2 | 4.3 | 3.6 | 1.1 | 90.9 | 92.0 | 98.0 |
| FA2G1S1 | 1.5 | 2.5 | 2.1 | 98.9 | 97.2 | 98.1 |
| A2G2S1 | 13.6 | 5.5 | 2.3 | 94.6 | 93.8 | 95.6 |
| FA2G2S1 | 2.1 | 2.2 | 3.5 | 99.2 | 98.4 | 95.6 |
| FA2BG2S1 | 8.7 | 3.6 | 12.1 | 93.1 | 95.2 | 68.6 |
| A2G2S2 | 27.1 | 5.9 | 10.1 | 93.7 | 79.1 | 92.0 |
| A2BG2S2 | 16.0 | 8.7 | 22.9 | 70.8 | 86.1 | 64.9 |
| FA2G2S2 | 5.5 | 6.1 | 21.3 | 96.9 | 93.9 | 57.5 |
| FA2BG2S2 | 7.8 | 7.6 | 35.2 | 93.5 | 92.2 | 18.8 |
| Average | 7.8 | 4.4 | 7.0 | 95.6 | 92.8 | 87.8 |
Repeatability was estimated by calculating CVs of 22 identical IgG standards and by calculating R2 of duplicates of 68 different samples.