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. 2018 Jul 26;5:71. doi: 10.3389/fmolb.2018.00071

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Copyright © 2018 Blakney, McKay and Shattock.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Design of positive, negative and non-structural protein (NSP) splitzicons encoding firefly luciferase. Key: CAP = m7G-cap, NSP1-4 = Non-structural proteins from the Venezuelan Equine Encephalitis Virus (VEEV), IRES = encephalomyocarditis virus internal ribosomal entry site, UTR = untranslated region, fLuciferase = firefly luciferase, black arrow = subgenomic promoter, 51nt CSE = conserved sequence element from NSP1, SG UTR = subgenomic UTR.